Journal
SMALL
Volume 3, Issue 9, Pages 1560-1565Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.200700148
Keywords
bioimaging; cells; labeling; peptides; quantum dots
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We present an integrin labeling method using functionalized quantum dots (QDs). Cyclic Arg-Gly-Asp (RGD) peptides and a biotin-streptavidin linkage are used to specifically couple individual QDs to integrins of living cells. The spacer distance between the RGD sequence and the QD surface is a crucial parameter to ensure specific binding to individual 03 integrins of osteoblast cells. Despite blinking, the position of single QDs is tracked with nanometer precision and localized diffusive behavior is observed. We show that blinking events do not prevent the acquisition of quantitative parameters from the QD trajectories.
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