Journal
FUNGAL GENETICS AND BIOLOGY
Volume 44, Issue 9, Pages 886-895Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.fgb.2006.12.001
Keywords
Aspergillus nidulans; proteomic analysis; osmoadaptation; glycerol biosynthesis; protein turnover; flux change; two-dimensional gel electrophoresis (2DE); matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF MS); peptide mass fingerprinting (PMF)
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The model filamentous fungus Aspergillus nidulans, when grown in a moderate level of osmolyte (+0.6 M KCl), was previously found to have a significantly reduced cell wall elasticity (Biotech Prog, 21:292, 2005). In this study, comparative proteomic analysis via two-dimensional gel electrophoresis (2DE) and matrix-assisted laser desorption ionization/time-of-flight (MALDI-TOF) mass spectrometry was used to assess molecular level events associated with this phenomenon. Thirty of 90 differentially expressed proteins were identified. Sequence homology and conserved domains were used to assign probable function to twenty-one proteins currently annotated as hypothetical. In osmoadapted cells, there was an increased expression of glyceraldehyde-3-phosphate dehydrogenase and aldehyde dehydrogenase, as well as a decreased expression of enolase, suggesting an increased glycerol biosynthesis and decreased use of the TCA cycle. There also was an increased expression of heat shock proteins and Shp1-like protein degradation protein, implicating increased protein turnover. Five novel osmoadaptation proteins of unknown functions were also identified. (C) 2006 Elsevier Inc. All rights reserved.
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