Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 389, Issue 1, Pages 197-205Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-007-1409-6
Keywords
dietary supplement; Standard Reference Material (SRM); bitter orange alkaloids; LC-API-ES-MS; synephrine; fluorinated stationary phase
Ask authors/readers for more resources
A liquid chromatographic atmospheric-pressure ionization electrospray mass spectrometry (LC-API-ES-MS) method has been developed for the determination of five bitter orange alkaloids (synephrine, octopamine, n-methyltyramine, tyramine, and hordenine) in bitter orange-containing dietary supplement standard reference materials (SRMs). The materials represent a variety of natural, extracted, and processed sample matrices. Two extraction techniques were evaluated: pressurized-fluid extraction (PFE) and sonication extraction. The influence of different solvents, extraction temperatures, and pH were investigated for a plant material and a processed sample. The LC method uses a new approach for the separation of highly polar alkaloids. A fluorinated, silica-based stationary phase separated the five alkaloids and the internal standard terbutaline in less than 20 min. This method enabled the determination of the dominant alkaloid synephrine and other minor alkaloids in a variety of dietary supplement SRMs.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available