A model system for targeted drug release triggered by biomolecular signals logically processed through enzyme logic networks

A new Sense-and-Act system was realized by the integration of a biocomputing system, performing analytical processes, with a signal responsive electrode. A drug-mimicking release process was triggered by biomolecular signals processed by different logic networks, including three concatenated AND logic gates or a 3-input OR logic gate. Biocatalytically produced NADH, controlled by various combinations of input signals, was used to activate the electrochemical system. A biocatalytic electrode associated with signal-processing biocomputing systems was electrically connected to another electrode coated with a polymer film, which was dissolved upon the formation of negative potential releasing entrapped drug-mimicking species, an enzyme-antibody conjugate, operating as a model for targeted immune-delivery and consequent prodrug activation. The system offers great versatility for future applications in controlled drug release and personalized medicine.