Journal
ANALYST
Volume 139, Issue 10, Pages 2265-2276Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4an00094c
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Funding
- Alfried Krupp von Bohlen und Halbach-Stiftung
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Liquid chromatography-mass spectrometry analysis of small molecules from biofluids requires sensitive and robust assays. Because of the very complex nature of many biological samples, efficient sample preparation protocols to remove unwanted components and to selectively extract the compounds of interest are an essential part of almost every bioanalytical workflow. This review describes the most common problems encountered during sample preparation, ways to optimize established sample preparation techniques and important recent developments to reduce or eliminate major interferents from biofluids.
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