Journal
ANALYST
Volume 139, Issue 2, Pages 488-494Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3an01670f
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Funding
- National 973 Program Project of China [2012CB932800]
- Shandong Provincial Natural Science Foundation [Q2008C08]
- Independent Innovation Foundation of Shandong University [IIFSDU-2012TS159]
- Shandong University [31370056431211, 31370070614018(2010TB018)]
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Nanoporous gold (NPG) based biosensors have been constructed by covalently immobilizing glucose oxidase (GOx) onto self-assembled monolayers (SAMs). With p-benzoquinone (BQ) as a mediator, diffusion behavior and amperometric biosensor performance are evaluated by electrochemical characterization. The enzyme modified electrodes are demonstrated to show a thickness-sensitive behavior. Compared with planar polycrystalline gold, the unique porous structure of NPG has also been characterized via an electrochemical surface reconstruction process. Single-crystal gold-like electrochemical behavior on NPG and a comprehensive understanding of its impacts on sensor performance have been proposed.
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