Journal
ANALYST
Volume 139, Issue 20, Pages 5155-5163Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4an01056f
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Funding
- Centre National de Recherche Scientifique (CNRS)
- Universite of Lille
- Nord Pas de Calais region
- Institut Universitaire de France (IUF)
- European Union
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We present in this work a simple and fast preparation method of a new affinity surface-assisted laser/desorption ionization mass spectrometry (SALDI-MS) substrate based on silicon nanostructures decorated with copper particles. The silicon nanostructures were fabricated by the metal-assisted chemical etching (MACE) method. Then, superhydrophilic areas surrounded by superhydrophobic regions were formed through hydrosilylation reaction of 1-octadecene, followed by local degradation of the octadecyl layer. After that, copper particles were deposited in the hydrophilic areas by using the electroless method. We have demonstrated that these surfaces were able to perform high selective capture of model His-tag peptide even in a complex mixture such as serum solution. Then, the captured peptide was detected by mass spectrometry at a femtomolar level without the need of organic matrix.
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