Cell supports of chitosan/hyaluronic acid and chondroitin sulphate systems.: Morphology and biological behaviour

Films and sponges of chitosan (CHI), chitosan/hyaluronic acid (CHI-HA) and chitosan/chondroitin sulphate (CHI-CHOS), were prepared by film deposition or lyophilization ( sponges), avoiding the formation of inter-polyelectrolyte complexes. The biological behaviour of the systems was analysed by studying the cell behaviour using a fibroblast cell line and standard biological MTT and Alamar Blue tests. The morphology of films, sponges and cell seeded samples was analysed by ESEM. The results obtained indicate that all the systems can be considered as good supports for cell adhesion and proliferation, but there is specific activation of the proliferative process in the presence of hyaluronic acid and chondroitin sulphate.