Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 856, Issue 1-2, Pages 285-293Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2007.06.010
Keywords
salmeterol; human plasma; matrix effects; liquid chromatography; mass spectrometry; solid-phase extraction
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Salmeterol is an inhaled bronchodilator drug used for treatment of asthma. Its concentrations in plasma are very low or undetectable by previously developed methods. The present paper describes a method for analysis of salmeterol in human plasma with 2.5 pg/mL lower limit of quantitation. Despite the basic character of the drug the method uses mixed mode anion-exchange solid phase extraction for sample preparation combined with a column switching approach to minimize matrix effects. Samples are separated and detected by LC/MS/MS. The method is easy to use, only requires 0.5 mL of plasma and was validated for use in bioanalytical applications. The method does not suffer from interference from co-administered fluticasone propionate. (C) 2007 Elsevier B.V. All rights reserved.
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