Journal
CHINESE CHEMICAL LETTERS
Volume 18, Issue 9, Pages 1107-1110Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cclet.2007.07.008
Keywords
ofloxacin (OFL); polyclonal antibody (pAb); competitive indirect enzyme-linked immunosorbent assay (CI-ELISA); colloidal gold-based immunochromatographic assay (CGIA)
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Two rapid, sensitive and reliable immunoassay methods, namely competitive indirect enzyme-linked immunosorbent assay (CI-ELISA) and colloidal gold-based inummochromatographic assay (CGIA), were developed to detect ofloxacin (OFL). The linear range of the CI-ELISA was from 0.5 to 128 ng/mL with a limit of detection (LOD) of 0.35 ng/mL. Good recoveries were obtained in analyzing simulated swine urine samples. The CGIA could accurately estimate OFL at concentrations as low as 10 ng/mL in less than 10 min, and test results were read visually without any instrument. (c) 2007 Ling Bo Qu. Published by Elsevier B.V. on behalf of Chinese Chemical Society, All rights reserved.
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