Journal
ANALYST
Volume 139, Issue 14, Pages 3563-3571Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3an02272b
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Funding
- Foundation for Polish Science within the FOCUS Program [F3/2010/P/2013]
- European Union [REGPOT-CT-2011-285949-NOBLESSE]
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The morphological changes of gold nanoparticles induced by T7 virus (bacteriophage) and the determination of its femtomolar concentration by a plasmonic method are presented. Carboxymethyl chitosan capped gold nanoparticles (CMC-AuNPs) are used as plasmonic probes and are synthesized by a simple one pot wet chemical method. HR-TEM images show that the spherical structure of the CMC-AuNPs is changed into chain-like nanostructures after the addition of T7 virus due to the strong coordination of CMC-AuNPs with T7. Since T7 capsids comprise a repeating motif of capsomers built from proteins that bind to the acid groups of chitosan, the conjugation of carboxymethyl chitosan-linked AuNPs with T7 virions enables colorimetric biosensing detection. The absorbance intensity (similar to 610 nm) increases in the concentration range of T7 from 2 x 10(-15) M to 2 x 10(-13) M and the detection limit is found to be 2 x 10(-15) M (2 fM). The present work demonstrates eco-friendly biopolymer stabilized AuNPs as potential nanomaterials for biosensing of viruses. Our method is very simple, low cost, selective and highly sensitive, and provides new insight into virus induced chain-like morphology of AuNPs.
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