Journal
ANALYST
Volume 138, Issue 24, Pages 7308-7315Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3an01266b
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Funding
- NSF [ECCS-0901154, DBI-0852701]
- NIH [R21EB014456, R01CA156674, R01CA90571, P01GM081621]
- UC Discovery/Abraxis BioScience Biotechnology Award [178517]
- Broad Center of Regenerative Medicine and Stem Cell Research at UCLA
- Div Of Biological Infrastructure
- Direct For Biological Sciences [1256178] Funding Source: National Science Foundation
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We report a 3D microfluidic pulsed laser-triggered fluorescence-activated cell sorter capable of sorting at a throughput of 23 000 cells per s with 90% purity in high-purity mode and at a throughput of 45 000 cells per s with 45% purity in enrichment mode in one stage and in a single channel. This performance is realized by exciting laser-induced cavitation bubbles in a 3D PDMS microfluidic channel to generate high-speed liquid jets that deflect detected fluorescent cells and particles focused by 3D sheath flows. The ultrafast switching mechanism (20 mu s complete on-off cycle), small liquid jet perturbation volume, and three-dimensional sheath flow focusing for accurate timing control of fast (1.5 m s(-1)) passing cells and particles are three critical factors enabling high-purity sorting at high-throughput in this sorter.
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