Enantioselective separation of dansyl-DL-amino acids and some racemates on click functionalized native α-cyclodextrin based sub-2 μm columns

The current work demonstrates that native alpha-cyclodextrin, anchored onto sub-2 mu m silica particles via click reactions and packed into a 5 cm column, was found to be effective for the resolution of 11 pairs of dansyl-DL-amino acids (DAAs) using ultra-high performance liquid chromatography (UHPLC). All DAAs were completely or partially separated on the column and the resolution achieved for 7 pairs of DAAs was significantly greater than 1.5. It was found that the buffer type exerted a profound impact on the separation. The effects of analyte substituents adjacent to the chiral center of analytes as well as operation conditions with respect to the separation efficiency were discussed. Five racemic compounds with single or double rings also got resolved on this short alpha-CD column to some extent.