Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 70, Issue 3, Pages 557-560Publisher
ELSEVIER
DOI: 10.1016/j.mimet.2007.06.012
Keywords
Phytophthora; recycled irrigation water; zoospore production
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A simple in-vitro 'wet-plate' method for mass-producing Phytophthora nicotianae zoospores at >= 1.0 x 10(6) zoospores/ml is described. Temperature critically affected zoospore production; 22 degrees C was optimum, while 36 degrees C was completely inhibitory. Zoospores being the most important propagule of P. nicotianae, temperature of recycled irrigation water may be manipulated to reduce diseases in irrigated nursery crops. (C) 2007 Elsevier B.V. All rights reserved.
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