Journal
BIOINFORMATICS
Volume 23, Issue 17, Pages 2331-2333Publisher
OXFORD UNIV PRESS
DOI: 10.1093/bioinformatics/btm343
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Funding
- NCRR NIH HHS [U54 RR021813] Funding Source: Medline
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We have developed a quick web-based application for designing conserved genomic PCR and RT-PCR primers from multigenome alignments targeting specific exons or introns. We used Pygr ( The Python Graph Database Framework for Bioinformatics) to query intervals from multigenome alignments, which gives us less than a millisecond access to any intervals of any genome within multigenome alignments. PRIMER3 was used to extract optimal primers from a gene of interest. QPRIMER creates an electronic genomic PCR image from a set of conserved primers as well as summary pages for primer alignments and products. QPRIMER supports human, mouse, rat, chicken, dog, zebrafish and fruit fly. Availability: http://www.bioinformatics.ucla.edu/QPRIMER/ Contact: leec@mbi.ucla.edu.
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