Journal
DEVELOPMENT
Volume 134, Issue 17, Pages 3145-3153Publisher
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/dev.001214
Keywords
Crk; Crkl; DOCK1; DOCK5; myoblast city; myoblast fusion; zebrafish
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Funding
- Medical Research Council [G0400100B, G0100151] Funding Source: researchfish
- Medical Research Council [G0100151] Funding Source: Medline
- MRC [G0100151] Funding Source: UKRI
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Myoblast fusion follows a defined sequence of events that is strikingly similar in vertebrates and invertebrates. Genetic analysis in Drosophila has identified many of the molecules that mediate the different steps in the fusion process; by contrast, the molecular basis of myoblast fusion during vertebrate embryogenesis remains poorly characterised. A key component of the intracellular fusion pathway in Drosophila is the protein encoded by the myoblast city (mbc) gene, a close homologue of the vertebrate protein dedicator of cytokinesis 1 (DOCK1, formerly DOCK180). Using morpholino antisense-oligonucleotide-mediated knockdown of gene activity in the zebrafish embryo, we show that the fusion of embryonic fast-twitch myoblasts requires the activities of Dock1 and the closely related Dock5 protein. In addition, we show that the adaptor proteins Crk and Crk-like (Crkl), with which Dock proteins are known to interact physically, are also required for myoblast fusion.
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