Journal
PLANT AND CELL PHYSIOLOGY
Volume 48, Issue 9, Pages 1385-1390Publisher
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcm106
Keywords
auto-isomerase; cyanobacteria; cyanobacteriocbrome; phycocyanobilin; phycoviolobilin; phytochrome
Categories
Ask authors/readers for more resources
Cyanobacteria have several putative photoreceptors (designated cyanobacteriochromes) that are related to but distinct from the established phytochromes. The GAF domain of the phototaxis regulator, PixJ, from a thermophilic cyanobacterium Thermosynechococcus elongatus BP-1 (TePixJ_GAF) is a cyanobacteriochrome which exhibits reversible photoconversion between a blue light-absorbing form (max = 433 nm) and a green light-absorbing form (max = 531 nm). To study the chromophore, we prepared TePixJ_GAF chromoprotein from heterologously expressed Synechocystis and performed spectral analysis after denaturation by comparing it with the cyanobacterial phytochrome Cph1 which harbors phycocyanobilin (PCB) as a chromophore. The results indicated that the chromophore of TePixJ is not PCB, but its isomer, phycoviolobilin (PVB). It is suggested that the GAIT domain of TePixJ has auto-lyase and auto-isomerase activities.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available