Journal
ANALYST
Volume 138, Issue 2, Pages 461-466Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2an36227a
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Funding
- 973 Program [2013CB933800]
- National Natural Science Foundation of China [21035003, 21227005, 21175076]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20113704130001]
- Program for Changjiang Scholars and Innovative Research Team in University
- Scientific Special Expenditure for Non-profit Public Industry of State Oceanic Administration [201105020]
- Science Foundation of China Postdoctoral [20100471568, 201104648]
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A novel strategy for selective and sensitive amperometric detection of lead ion (Pb2+) was proposed based on target-induced strand release. The underlying gold electrode was pre-modified with dendritic gold nanoparticles by direct electrodeposition to afford increased electrode surface area for immobilization of thiol group-containing capture DNA molecules. The hybridization of the capture DNA molecules with Pb2+-specific aptamer molecules to form a DNA duplex, into which methylene blue was intercalated, induced measurable electrochemical signal. Upon addition of Pb2+, it could specifically bind to its aptamer to form Pb2+-stablized G-quadruplex and induce the aptamer strand to release from the electrode surface into solution, accompanied by the release of intercalated MB responsible for significant signal reduction. The fabricated biosensor showed a linear response to the logarithm of Pb2+ concentration over the range of 1.0 x 10(-10) M to 1.0 x 10(-7) M with a detection limit of 7.5 x 10(-11) M. In addition, this strategy afforded an exquisite selectivity for Pb2+ against other metal ions. The excellent sensitivity and selectivity show good potential for Pb2+ detection in real environmental samples.
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