Journal
ANALYST
Volume 137, Issue 20, Pages 4760-4765Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2an35762c
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Funding
- [22680041]
- Grants-in-Aid for Scientific Research [22680041] Funding Source: KAKEN
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A split SNAP-tag protein complementation assay was developed for visualization of protein-protein interactions in living cells. Split SNAP-tagms, fragments of divided SNAP-tag between amino acid residues 91 and 92, were fused to proteins that can interact with each other. After incubation with a fluorescent SNAP-tag substrate, cells that expressed split SNAP-tag fusion proteins generated fluorescent signals when these proteins interacted. Moreover, by combination with the split CLIP-tag (SNAP-tag mutant), simultaneous labeling was achieved. This split SNAP-tag labeling method should be a useful tool for visualization of protein-protein interaction processes.
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