Journal
ANALYST
Volume 137, Issue 3, Pages 735-740Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2an15965a
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Funding
- Natural Science Foundation of China [20805036, 21035005, 21175111]
- Program for New Century Excellent Talents in University [NCET-10-0699]
- Fundamental Research Fund for the Central University [XDJK2010C065]
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Multianalyte immunoassay in a single run is often necessary to monitor or quantitate several components in a complex sample matrix for various purposes. In this paper we present a novel, individually addressable electrode array for sequential electrochemiluminescent (ECL) immunoassay using a non-array detector. An immunosensor array was fabricated by site-selectively immobilizing multiple antigens on different electrodes. With a competitive immunoassay format, the amounts of the bound Ru(bpy)(3)(2+) derivative labeled antibodies decreased with the increase of the antigens in the sample, and the ECL signals from different immunosensors were collected in turn by a photomultiplier with the aid of a home-made single-pore-three-throw switch. Using human IgG and rat IgG as model analytes, this multianalyte immunoassay showed detection limits down to 8.9 and 7.2 ng mL(-1) for them, respectively. The results for real sample analysis demonstrated that this strategy can provide a simple, sensitive, low-cost and high-throughput ECL immunosensor array for clinical diagnosis.
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