Journal
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
Volume 293, Issue 3, Pages E713-E725Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpendo.00194.2007
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Funding
- NHLBI NIH HHS [P50 HL-081089, HL-51387, HL-65192] Funding Source: Medline
- NIDDK NIH HHS [U24 DK-59637, U24 DK059637] Funding Source: Medline
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Macrophage TNF-alpha contributes to insulin resistance and hepatic steatosis in diet-induced obesity. Am J Physiol Endocrinol Metab 293: E713-E725, 2007. First published June 19, 2007; doi:10.1152/ajpendo.00194.2007. - Obesity is commonly associated with development of insulin resistance and systemic evidence of inflammation. Macrophages contribute to inflammatory amplification in obesity and may contribute directly to insulin resistance and the development of nonalcoholic fatty liver disease through the production of inflammatory cytokines, including tumor necrosis factor (TNF)-alpha. To test this hypothesis, we transplanted male wild-type ( WT) and TNF-alpha deficient (KO) mice with either TNF-alpha- sufficient ( TNF-alpha(+/+)) or TNF-alpha-deficient (TNF-alpha(-/-)) bone marrow. After consuming a high-fat diet for 26 wk, metabolic and morphometric characteristics of the animals were analyzed. While there were no differences in terms of relative weight gain, body composition analysis yielded a lower relative adipose and higher relative lean mass in mice lacking TNF-alpha, which was partially explained by reduced epididymal fat pad and liver weight. TNF-alpha(+/+) 3KO mice exhibited enhanced insulin sensitivity compared with that observed in TNF- alpha(+/+) 3KO mice; remarkably, no protection against insulin resistance was provided by transplanting TNF-alpha(-/-) bone marrow in WT mice compared with TNF-alpha(+/+) -> WT. The preserved insulin sensitivity seen in TNF-alpha(+/+) 3KO mice provided protection against the development of hepatic steatosis. Taken together, these data indicate that macrophage-derived TNF- alpha contributes to the pattern and extent of fat accumulation and insulin resistance in diet-induced obesity; however, this contribution is negligible in the presence of host-derived TNF-alpha.
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