Halobacillus campisalis sp nov., containing diaminopimelic acid in the cell-wall peptidoglycan, and emended description of the genus Halobacillus

A Gram-positive or variable, motile and coccoid or oval-shaped bacterial strain, ASL-17(T), was isolated from a marine solar saltern of the Yellow Sea in Korea and its exact taxonomic position was investigated by a polyphasic approach. Strain ASL-17(T) grew optimally at pH 7.0-8.0 and 37 degrees C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ASL-17(T) is most closely affiliated phylogenetically to the genus Halobacillus. Strain ASL-17(T) exhibited 16S rRNA gene sequence similarity values of 97.7-98.6% to the type strains of recognized Halobacillus species. Interestingly, strain ASL-17(T) had cell-wall pepticloglycan based on meso-diaminopimelic acid, unlike other Halobacillus species. It contained MK-7 as the predominant menaquinone. The major fatty acids were ante iso-C-15:0, ante iso-C-17:0 and iso-C-16:0. The DNA G + C content was 42.1 mol%. DNA-DNA relatedness data and differential phenotypic properties demonstrated that strain ASL-17(T) can be differentiated from recognized Halobacillus species. On the basis of phenotypic, chemotaxonomic, phylogenetic and genetic data, strain ASL-17(T) represents a novel species of the genus Halobacillus, for which the name Halobacillus campisalis sp. nov. is proposed. The type strain is ASL-17(T) (=KCTC 13144(T)=CCUG 54360(T)).