Journal
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
Volume 55, Issue 9, Pages 877-883Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1369/jhc.7A7185.2007
Keywords
lycopene; breast cancer cell lines; immunohistochemistry; RT-QPCR; RAR alpha; RAR beta; Cx43
Categories
Ask authors/readers for more resources
Lycopene, the major carotenoid found in tomatoes, is a potent antioxiclant associated with the prevention of degenerative diseases such as breast cancer. This effect could be due to the interaction between lycopene and retinoic acid receptors as well as the stimulation of gap junction communication and synthesis of connexin 43. The expression of the RARa, RARP, and Cx43 proteins was analyzed using immunohistochemistry in two breast cancer cell lines, MCF-7 and MDA-MB-231, and in a fibrocystic dystrophy cell line, MCF-10a, after a 48-hr exposure to 10 mu M lycopene. A real-time quantitative PCR analysis was then performed to measure mRNA expression. RARa and Cx43 expression were increased at both mRNA and protein levels in two breast cell lines.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available