4.6 Article

Colorimetric sensing of trace UO22+ by using nanogold-seeded nucleation amplification and label-free DNAzyme cleavage reaction

Journal

ANALYST
Volume 137, Issue 8, Pages 1866-1871

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c2an00039c

Keywords

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Funding

  1. National Natural Science Foundation of China [21165005, 21075023]
  2. Natural Science Foundation of Guangxi [0991021Z]
  3. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University)
  4. Ministry of Education [1101Z005]
  5. Guangxi Water Conservancy

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In pH 4.4 HAC-NaAC buffer solution at 80 degrees C, nanogold particles (NG) strongly enhanced the slow, colored reaction of Ag(I)-gallic acid to form nanosilver particles, which exhibited a strong surface plasmon resonance (SPR) absorption peak at 460 nm, but the aggregated nanogold particles (ANG) exhibited a weak enhancement. The increased absorption value at 460 nm was linear to the NG concentration in the range of 3.6-72.5 ng mL(-1) Au. In pH 5.5 MES buffer solution at 80 degrees C, single-stranded substrate DNA and DNAzyme hybridize to form double-stranded DNA (dsDNA). The presence of uranyl (UO22+) resulted in cleavage of the substrate DNA of dsDNA, releasing a short, single-stranded DNA that can be adsorbed onto the NG and protect them from aggregation; those un-adsorbed NG were aggregated to ANG. As the UO22+ concentration increased, more short, singlestranded DNA were released, and more NG were protected by the cleavage of substrate single-strand DNA, so the colored particle reaction and the absorption value at 460 nm enhanced linearly. On those grounds, 0.083-0.67 nmol L-1 UO22+ can be detected rapidly by this colorimetric sensing assay, with a detection limit of 0.04 nmol L-1.

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