Journal
ANALYST
Volume 137, Issue 18, Pages 4235-4240Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2an35753d
Keywords
-
Categories
Funding
- National Natural Science Foundation of China [21175138, 20935005]
- Ministry of Science and Technology of China [2007CB714504]
- Chinese Academy of Sciences
Ask authors/readers for more resources
A novel amino acid ionic liquid (AAIL) with L-ornithine (L-Orn) as anion was successfully synthesized, and subsequently applied as an available chiral ligand coordinated with Zn(II) in a chiral ligand exchange capillary electrophoresis (CLE-CE) system for the enantioseparation of dansyl amino acids (Dns-D,L-AAs). The influence of key parameters, such as buffer pH, concentration ratio of Zn(II) to ligand and complex concentration, was investigated in detail. Eleven pairs of Dns-D, L-AAs enantiomers were baseline separated and three pairs were partly separated under the optimum conditions. For exploring its potential application, the quantitative features of this proposed method were studied. Good linearity (r(2) = 0.999) and favorable repeatability (RSD <= 3.4%) were obtained by using Dns-D,L-Met as the test analyte. Finally, this method was employed to investigate the inhibition efficiency of Damino acid oxidase (DAAO) inhibitors, which may pave a new way for the high-throughput screening of enzyme inhibitors and relevant drug discovery.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available