Journal
NANO LETTERS
Volume 7, Issue 9, Pages 2618-2626Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl0709930
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Funding
- NIBIB NIH HHS [R01 EB 000364, R01 EB004343] Funding Source: Medline
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Cholera toxin subunit B (CTB)-quantum dot conjugates were developed for labeling mammalian cells. The conjugates were internalized by all tested cell lines into small vesicles dispersed throughout the cytoplasm, while commercially available polyarginine conjugates rapidly accumulated in large perinuclear endosomes. Although a large proportion of CTB conjugates eventually also accumulated in perinuclear endosomes, this accumulation required several days, and even then many CTB conjugated quantum dots remained in small vesicles dispersed throughout the cytoplasm. Thus CTB conjugates are a practical alternative to polyarginine conjugates for the general labeling of mammalian cells.
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