Journal
ANALYTICAL CHEMISTRY
Volume 79, Issue 17, Pages 6507-6514Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac070394d
Keywords
-
Categories
Ask authors/readers for more resources
Multifluorescent silica nanoparticles were synthesized by the Stober method using conjugates of (3-aminopropyl)-triethoxysilane and fluorescent dye-N-hydroxysuccinimide esters. The nanoparticles containing the fluorescent dyes were well dispersed and showed high, stable, and tunable fluorescence intensities. In addition, we prepared multifluorescent silica nanoparticles containing two kinds of fluorescent dyes and used the nanoparticles in biological applications. Flow cytometry analysis showed high and tuned fluorescence and multiple fluorescences from single nanoparticles with diameters of similar to 400 nm. Fluorescence microscopy analysis also showed high and tuned fluorescence, as well as multiple fluorescences from single nanoparticles and from cells labeled with multifluorescent silica nanoparticles. The intracellular distribution of nanoparticles was evaluated by confocal microscopy and electron microscopy. We discuss the advantages and demonstrate the usefulness of our nanoparticles in relation to commercially available fluorescent narroparticles including quantum dots.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available