Journal
ANALYST
Volume 136, Issue 18, Pages 3698-3706Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1an15108h
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Funding
- NSF China [20802060, 21072162, 30830092, 30921005, 91029304, 81061160512]
- Xiamen University [2011121020]
- 973 program [2009CB522200]
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The parameters of intracellular acidic compartments are altered in apoptosis, autophagy, cancer metastasis, etc. Low background and selective staining of lysosomes and autophagosomes was achieved with N-(rhodamine 6G)-lactam-ethylenediamine (R6G-EDA) which fluoresces in acidic vesicles via pH mediated ring opening of the intramolecular lactam. Long retention time of R6G-EDA in lysosomes and its exceptional stability against photo-bleaching allow full time tracking of lysosome morphology changes in tumor necrosis factor-alpha (TNF-alpha) triggered cell death, suggesting its utility for acidic vesicle studies in cell biology.
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