Journal
ANALYST
Volume 136, Issue 15, Pages 3136-3141Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1an15224f
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Funding
- NSFC
- State Basic Program
- Chinese Academy of Sciences
- Transregio Project [TRR61]
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Gold nanoparticles (Au-NPs) with negative charges aggregate in the presence of Arg(6) due to electrostatic interactions resulting in the red-shift of the plasmon absorption. But, after incubation of Arg(6) with trypsin the aggregation of Au-NPs can be prohibited. Accordingly, a newly designed-Au-NPs based colorimetric assay method for trypsin activity is established. Trypsin with a concentration as low as 1.6 ng mL(-1) can be assayed with this new colorimetric assay. This colorimetric label-free assay for trypsin can be performed in aqueous solution and both Au-NPs and Arg(6) are easily accessible. Thus, this assay method is useful for screening inhibitors of trypsin.
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