Journal
PEPTIDES
Volume 28, Issue 9, Pages 1706-1712Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2007.04.007
Keywords
pituitary adenylate cyclase activating polypeptide (PACAP); vasoactive intestinal peptide (VIP); cyclic AMP; neurons; astrocytes; rat; hypoxia
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Pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal peptide (VIP) concentration (0.001-1000 nM)-dependently stimulated cyclic AMP production in rat primary neuronal and glial cell (astrocyte) cultures. The actions of both peptides were much more pronounced in astrocytes than in neuronal cultures. Stimulatory effects of PACAP and VIP on cyclic AMP formation were significantly smaller in cell cultures subjected to 24 h lasting hypoxic conditions, induced either chemically (100 mu M cobalt chloride) or by low 3% oxygen hypoxia, compared to the normoxic condition (95% air and 5% CO2). This picture contrasted with the effects of forskolin that were similar under normoxic and hypoxic conditions. It is suggested that hypoxia leads to changes in PACAP-and VIP-driven cyclic AMP-dependent signaling in the rat brain by influencing molecular processes likely occurring at the level of receptor protein or receptor-Gs protein coupling. (c) 2007 Published by Elsevier Inc.
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