Journal
ANALYST
Volume 136, Issue 24, Pages 5241-5246Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0an01031f
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Funding
- Estonian Science foundation [7127]
- Ministry of Education and Science of Estonia [SF0180061s08]
- iMERA-PLUS [T2J10]
- EMRP within EURAMET
- European Union
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A simple method to identify and determine Selenomethionine (SeMet) and Selenomethylselenocysteine (Se-MeSeCys) with diethyl ethoxymethylenemalonate (DEEMM) derivatization and LC-ESI-MS/MS determination was developed. Separation of SeMet and Se-MeSeCys was achieved in 15.3 minutes. The calibration graph was linear in the range of 0.32 pmol to 49 pmol for SeMet and 0.34 pmol to 40 pmol for Se-MeSeCys. To prevent oxidation of SeMet, 2-mercaptoethanol was introduced to the calibration solutions. Detection limits were 0.1 pmol, which are comparable to LC-ICP-MS analysis. The developed method therefore offers an alternative to LC-ICP-MS offering similar sensitivity and additionally allows identification. The method was used to determine Se-MeSeCys and SeMet in onion samples.
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