Journal
HAEMATOLOGICA
Volume 92, Issue 9, Pages 1208-1215Publisher
FERRATA STORTI FOUNDATION
DOI: 10.3324/haematol.11446
Keywords
rejection; humoral; immunogenicity; cross match
Categories
Funding
- Swedish Cancer Society [4562-B05-05XCC]
- Children's Cancer Foundation [05/007]
- Swedish Research Council [K2006-32X-14716-04-1, K2005-32P-15457-01A]
- Tobias Foundation
- Cancer Society in Stockholm
- Swedish Society of Medicine
- Stockholm County Council
- Sven and Ebba-Christina Hagbergs Foundation
- Karolinska Institutet
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Background and Objectives Mesenchymal stromal cells (MSC) may be used in cellular therapy to treat graft-versus-host-disease and autoimmune disorders, and in regenerative medicine. Preliminary data suggest limited cellular allogeneic rejection, but less is known about humoral responses. The objective of this study was to investigate whether antibodies against MSC were present after hematopoietic stem cell transplantation (HSCT) including treatment with HLA matched or mismatched allogeneic MSC. Design and Methods Twelve patients were evaluated using flow cytometric cross matches (FCXM) and enzyme-linked immunosorbent assays. Expression of blood group antigens, regarded as alloantigens giving rise to humoral alloimmunity, on MSC were explored using flow cytometry and immunofluorescence. Results Three of 12 patients exhibited late positivity in the FCXM. In absorption studies, antibodies directed against fetal calf serum (FCS), a component of the MSC culture medium, were identified. Healthy individuals expressed varying levels of anti-FCS antibodies and the same pattern was seen in immunosuppressed HSCT patients. MSC did not express blood group antigens. The patients with positive FCXM are alive and well. Interpretation and Conclusions We have shown that immunosuppressed patients can exhibit anti-FCS antibodies, but no alloantibodies, which may bind to MSC. These antibodies seem clinically insignificant.
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