Journal
PLANT BIOTECHNOLOGY JOURNAL
Volume 5, Issue 5, Pages 636-645Publisher
BLACKWELL PUBLISHING
DOI: 10.1111/j.1467-7652.2007.00268.x
Keywords
3-ketoacyl-CoA synthase; Crambe abyssinica; FAE expression; erucic acid; Arabidopsis thaliana; Brassica carinata
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A genomic fatty acid elongation 1 (FAE1) clone was isolated from Crambe abyssinica. The genomic clone corresponds to a 1521 -bp open reading frame, which encodes a protein of 507 amino acids. In yeast cells expression of CrFAE led to production of new very long chain monounsaturated fatty acids such as eicosenoic (20 : 1(Delta 11)) and erucic (22 : 1(Delta 13)) acids. Seed-specific expression in Arabidopsis thaliana resulted in up to a 12-fold increase in the proportion of erucic acid. On the other hand, in transgenic high-erucic Brassica carinata plants, the proportion of erucic acid was as high as 51.9% in the best transgenic line, a net increase of 40% compared to wild type. These results indicate that the CrFAE gene encodes a condensing enzyme involved in the biosynthesis of very long-chain fatty acids utilizing monounsaturated and saturated acyl substrates, with a strong capability for improving the erucic acid content.
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