Journal
ANALYST
Volume 136, Issue 18, Pages 3753-3757Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1an15198c
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Funding
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP, Sao Paulo, Brazil)
- Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG, Belo Horizonte, Brazil) [CDS-APQ-01323-09, CDS-APQ-01612-10]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Brasilia, Brazil)
- Ministerio de Ciencia e Tecnologia (INOMAT)
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES, Brasilia, Brazil)
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The association of solid phase extraction with molecularly imprinted polymers (MIP) and electrospray ionization mass spectrometry (ESI-MS) is applied to the direct extraction and quantitation of benzodiazepines in human plasma. The target analytes are sequestered by MIP and directly analyzed by ESI-MS. Due to the MIP highly selective extraction, ionic suppression during ESI is minimized; hence no separation is necessary prior to ESI-MS, which greatly increases analytical speed. Benzodiazepines (medazepam, nitrazepam, diazepam, chlordiazepoxide, clonazepam and midazolam) in human plasma were chosen as a proof-of-principle case of drug analyses by MIP-ESI-MS in a complex matrix. MIP-ESI-MS displayed good figures of merits for medazepam, nitrazepam, diazepam, chlordiazepoxide and midazolam, with analytical calibration curves ranging from 10 to 250 mu g L-1 (r > 0.98) with limit of quantification <10 mu g L-1 and acceptable within-day and between-day precision and accuracy.
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