Journal
ANALYST
Volume 136, Issue 8, Pages 1632-1636Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0an00880j
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Funding
- NSFC [50503006, 20801014, 20735002]
- Science Foundation of Fujian Province [2008J0149, JA10017]
- P. R. China
- Fuzhou University
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A simple, selective and sensitive turn-on fluorescent sensor for the detection of mercury(II) ion was developed using Sybr Green I as the signal reporter and SWCNTs as the quencher. Due to the affinity of SWCNTs towards ssDNA and organic dye, Sybr Green I, thymine-rich ssDNA and SWCNTs could form a self-assembly of three components, resulting in fluorescence quenching. Upon addition of another thymine-rich ssDNA and mercury(II) ion, formation of dsDNA via T-Hg2+-T base pairs enabled Sybr Green I to intercalate into the dsDNA, resulting in the restoration of fluorescence. SWCNTs were found to reduce the background signal and improve the analytical sensitivity. A linear relationship between the fluorescence intensity and the concentration of mercury(II) ion was observed in the range of 20-1250 nM (R = 0.9985) with a detection limit of 7.9 nM. The proposed method was applied to detect mercury(II) ion in tap water samples with good results.
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