Journal
ANALYST
Volume 135, Issue 6, Pages 1370-1374Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b927012d
Keywords
-
Categories
Funding
- BMBF [05KS7PC2]
- DFG [HA2394/12-2]
Ask authors/readers for more resources
Confocal Raman microspectroscopy was used to investigate human sperm cells. Raman mapping with a 532 nm excitation laser allowed to unambiguously characterize the nucleus, the neck, and, in particular, the mitochondria-rich middle piece of a human sperm cell. The effect of ultraviolet radiation on different organelles of the sperm was quantified by localized spectral Raman signatures obtained within milliseconds. Chemical changes within the sub-cellular structure of the sperm cells were recorded as a function of ultraviolet light exposure time, showing the proof-of-principle that Raman microspectroscopy can be a fast diagnostic method for detecting the mitochondrial and motility status of human spermatozoa.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available