Journal
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
Volume 19, Issue 5, Pages 486-491Publisher
AMER ASSOC VETERINARY LABORATORY DIAGNOSTICIANS INC
DOI: 10.1177/104063870701900505
Keywords
capripoxvirus; immunostaining; ovine testis cells (OA3.Ts)
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An ovine testis cell line (OA3.Ts) was evaluated and compared with primary lamb kidney (LK) cells for its utility in capripoxvirus propagation and titration. A comparison of OA3.Ts cell growth kinetics and morphology at low (< 33) and high (34-36) passage levels indicated a difference in both characteristics. However, viral titers determined in low and high passage OA3.Ts cells were comparable with those obtained using LK cells. Capripoxvirus infection of OA3.Ts and LK cells resulted in a similar cytopathic effect, which allowed for the detection of discrete viral plaques following immunostaining with capripoxvirus-specific antiserum.
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