Journal
GENETICS
Volume 177, Issue 1, Pages 255-265Publisher
GENETICS SOCIETY AMERICA
DOI: 10.1534/genetics.107.076315
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Funding
- Intramural NIH HHS Funding Source: Medline
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Schizosaccharomyces pombe cells can switch between two mating types, plus (P) and minus (M). The change in cell type occurs due to a replication-coupled recombination event that transfers genetic information from one of the silent-donor loci, mat2P or mat3M, into the expressed mating-type determining mail locus. The mail locus can as a consequence contain DNA encoding either Por Minformation. A molecular mechanism, known as synthesis-dependent strand annealing, has been proposed for the underlying recombination event. A key feature of this model is that only one DNA strand of the donor locus provides the information that is copied into the mat1. Here we test the model by constructing strains that switch using two different mutant P cassettes introduced at the donor loci, mat2 and mat3. We show that in such strains wild-type P-cassette DNA is efficiently generated at mail through heteroduplex DNA formation and repair. The present data provide an in vivo genetic test of the proposed molecular recombination mechanism.
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