Journal
ANALYST
Volume 135, Issue 10, Pages 2661-2667Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0an00221f
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Funding
- Alexander von Humboldt-Foundation
- Federal Ministry of Education and Research (BMBF)
- Government of the Balearic Islands through FEDER
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A simple and sensitive fluorescence immunoassay for the detection of aflatoxin B-1 (AFB(1), as a model compound) in food was developed using AFB(1)-bovine serum albumin conjugate (AFB(1)-BSA)-functionalized magnetic beads as immunosensing probes. The recognition elements were prepared by doping of rhodamine B (RB) fluorophore into silica nanoparticles followed by immobilization of monoclonal anti-AFB(1) antibodies on the silica shell. Based on a competitive-type immunoassay format, the assay was performed both in low-binding polypropylene 96-well microtiter plates (MTPs) and in an automated sequential injection (SI) format. Similar detection limit (LOD) of 0.2 ng mL(-1) vs. 0.1 ng mL(-1) but narrower dynamic working linear range of 0.5-7 ng mL(-1) vs. 0.5-30 ng mL(-1) was obtained toward AFB(1) standards with the flow setup compared to the MTP format. Intra-batch assay precision was substantially improved (<= 5.3% vs. <= 8.7%) by resorting to the SI manifold. The proposed method features unbiased identification of negative (blank) and positive samples. No significant differences at the 95% confidence level were encountered in the analysis of naturally contaminated peanut samples between the proposed immunoassay and liquid chromatography for determination of AFB(1).
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