Journal
ANALYST
Volume 135, Issue 8, Pages 2069-2073Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0an00215a
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Funding
- NSFC [20805038]
- MOE [200803841013]
- National Basic Research Program of China [2007CB935603, 2010CB732402]
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Based on Exonuclease III (Exo III) and displacing probes, we have developed a Cyclic Enzymatic Amplification Method (CEAM) for sensitive and selective detection of nucleic acids. In this design, the displacing probe is non-fluorescent on its own and cannot be digested by Exo III until displacement hybridization by a target sequence, leading to release of free non-quenched fluorophore. Because a single target sequence can lead to the release and digestion of numerous fluorophore strands from the displacing probe, a remarkable signal amplification is achieved. With this method, DNA can be detected in the picomolar range with a high selectivity and within less than 20 min.
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