4.6 Article

Infrared microspectroscopy of live cells in aqueous media

Journal

ANALYST
Volume 135, Issue 12, Pages 3227-3232

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c0an00548g

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Funding

  1. NIH [CA 090346]
  2. NATIONAL CANCER INSTITUTE [R01CA090346] Funding Source: NIH RePORTER

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Fourier Transform Infrared (FTIR) spectroscopic measurements of individual, live HeLa cells in culture and buffer media are presented. Spectral data were acquired using a newly designed live cell chamber developed in the authors' laboratory. Data were processed using MATLAB-based routines that correct for the overcompensation of water encountered during live cell measurements in aqueous samples. Data presented are from live cells monitored over an extended period of time as well as a comparison of live cells exposed to perturbing conditions.

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