Journal
ANALYST
Volume 135, Issue 7, Pages 1519-1530Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0an00075b
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Funding
- National Basic Research Program of China (973 Program) [2010CB933504]
- National Cancer Institute [P30 CA021765]
- American Lebanese Syrian Associated Charities (ALSAC)
- St. Jude Children's Research Hospital
- NATIONAL CANCER INSTITUTE [P30CA021765] Funding Source: NIH RePORTER
- NATIONAL CENTER FOR RESEARCH RESOURCES [P20RR016457] Funding Source: NIH RePORTER
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A significant increase in biomedical applications of nanomaterials and their potential toxicity demands versatile analytical techniques to determine protein-nanoparticle (NP) interactions. These diverse analytical techniques are reviewed. Spectroscopic methods play a significant role in studying binding affinity, binding ratio, and binding mechanisms. To elucidate NP-proteome interactions, chromatography and electrophoresis techniques are applied to separate NP-bound proteins and matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to identify these proteins. Since NP-protein binding is a dynamic event, surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) are methods of choice to study the kinetics of NP-protein binding.
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