Journal
ANALYST
Volume 135, Issue 3, Pages 603-607Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b920998k
Keywords
-
Categories
Funding
- MEST [2009-0080734]
- MOEHRD [KRF-2007-357-C0072]
- SRC [R11-2005-008-060020]
- Seoul RBD [10543]
- BK21 fellowship
Ask authors/readers for more resources
This study describes the development and characterization of a novel dendritic signal amplification strategy. It relies on the use of two different Ru(bpy)(3)(2+)-doped silica nanoparticles (Probe(1,2)RSNP and Probe(2c)RSNP) coated with complementary DNAs, which can be simply and conveniently self-assembled to build sandwich-type dendritic architectures on a gold grid. The performance of this dendritic amplification route was demonstrated in conjunction with the electrogenerated chemiluminescent (ECL) detection of the target DNA. Compared to normal amplification, dendritic amplification allowed a 5-fold enhancement of the ECL signals. The higher sensitivity allowed by the dendritic amplification route was attributed to the hybridization between the DNA (Probe(2)DNA) on Probe(1,2)RSNP (normal amplification) and the complementary DNA (Probe(2c) DNA) on the additional Probe(2c)RSNP. As low as 1 fM of 22-bp-long target DNA was clearly detected. The experimental results demonstrated that the ECL intensity achieved through dendritic amplification showed a good linear relationship with the concentration of the target DNA over a wide linear range (10 fM similar to 10 pM).
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available