Journal
ANALYST
Volume 135, Issue 10, Pages 2653-2660Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0an00386g
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Funding
- National Natural Science Foundation of China [39670298]
- Science Foundation of Shantou University [YR09009]
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Anticancer drugs which selectively interact with DNA can change DNA's conformation and inhibit the duplication or transcription of DNA. An instrument-based assay for directly screening DNA-targeted anticancer drugs using resonance light scattering (RLS) technique with a common spectrofluorometer was proposed. To monitor the proposed screening method, the interactions between three anticarcinogens (Adriamycin (ADM), Bleomycin A (BLMA), Actinomycin D (ACTD)) and DNA were studied. The sequence of binding constants for the three anticarcinogens obtained from RLS spectra is: K-RLS (ACTD, 9.43 x 10(5) L mol(-1)) > K-RLS (ADM, 6.67 x 10(5) L mol(-1)) > K-RLS (BLMA, 8.88 x 10(3) L mol(-1)) and of binding numbers is: N-RLS (ACTD, 3.36 mmol g(-1)) < N-RLS (ADM, 3.81 mmol g(-1)) < N-RLS (BLMA, 57.44 mmol g(-1)). From the results we got the sequence of combination intensity between these three drugs and DNA as follows: ACTD-DNA > ADM-DNA > BLMA-DNA, which was completely consistent with drug activity. The conclusion indicated that the present method was direct, rapid, reliable and was another important innovation of the application of RLS technique.
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