Journal
ANALYST
Volume 134, Issue 9, Pages 1826-1833Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b823360h
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Funding
- National Basic Research Program of China [2007CB936000]
- National Natural Science Funds for Distinguished Young Scholar [20725518]
- Major Program of National Natural Science Foundation of China [90713019]
- National Natural Science Foundation of China [20575036]
- Important Project of Natural Science Foundation of Shandong Province in China [Z2006B09]
- Ministry of Education [20060445002]
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A new fluorescent and colorimetric Cu2+ probe was synthesized, and realized optical imaging in RAW264.7 macrophages. The design strategy was based on a change in structure between spirocyclic (non-fluorescent) and ring-open ( fluorescent) forms of rhodamine-based dyes, and its crystal structure was presented to explain the binding mode. Upon treatment with Cu2+, the weakly fluorescent probe exhibited a strong fluorescence response, high selectivity and was quantitative for Cu2+ under physiological conditions. In addition, the off-on-type fluorescent change upon the addition of Cu2+ was also applied in bioimaging.
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