Journal
ANALYST
Volume 133, Issue 4, Pages 444-447Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b802360n
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A simple procedure for the bio-conjugation of amphiphilic polymer-coated quantum dots with antibodies is described. The formed bio-conjugates are purified from an excess of free antibodies and of free nanoparticles by size-exclusion HPLC and then characterized by fluorescence emission and MALDI-TOFMS. The applicability of the approach is demonstrated for aflatoxin B1 detection.
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