Journal
FEBS LETTERS
Volume 581, Issue 22, Pages 4241-4247Publisher
WILEY
DOI: 10.1016/j.febslet.2007.07.070
Keywords
cardiomyocyte; force direction; mechanotransduction; focal adhesion kinase; refractory period; nucleo-cytoplasmic transport
Funding
- NHLBI NIH HHS [F31 HL077995, HL 62426, HL 64956, HL 077995, P01 HL062426, P01 HL062426-070002, R01 HL064956] Funding Source: Medline
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The effect of interval, direction and rate of strain on mechanotransduction in neonatal rat cardiomyocytes is determined for focal adhesion kinase (Y397pFAK), extracellular signal-regulated kinase ERK1/2 (Tbr(183)/Tyr(185)) and paxillin (pY31) and phosphorylation time courses to 10% strain assessed. Cells are non-responsive at 5 min but recover at 15 min (P < 0.03) with FAK nuclear translocation by 30 min. Cyclic biaxial strain increased phosphorylation from slower to faster rates (P < 0.05). Uniaxial strain to groove-aligned myocytes increased FAK and ERK1/2 phosphorylation transversely more than longitudinalh, (P < 0.05). Mechanotransduction may have a refractory period of 5 min and differentiate directions and rates of strain. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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