Journal
VACCINE
Volume 25, Issue 36, Pages 6646-6654Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2007.06.029
Keywords
dengue virus serotype 2 envelope domain III protein; neutralizing antibody; tobacco mosaic virus
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The envelope protein of dengue virus is the major protein involved in host cell receptor binding for viral entry and induction of immunity. A gene fragment encoding domain III of the dengue 2 envelope protein (D2EIII, amino acids 298-400) was successfully expressed in Nicotinana benthamiana plant using a tobacco mosaic virus (TMV)-based transient expression system. The N-terminal 5' untranslated region-omega sequence located upstream of D2EIII increased protein production in infected plant tissues. The recombinant protein was reactive with anti-D2EIII polyclonal and anti-His tag antibodies. The intramuscular immunization of mice with D2EIII induced the production of the anti-dengue virus antibody. The induced antibody demonstrated neutralizing activity against dengue type 2 virus. The result indicates that the TMV expression system produces the dengue virus antigen in plant, which possesses appropriate antigenicity and immunogenicity. (c) 2007 Elsevier Ltd. All rights reserved.
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