Journal
FEBS LETTERS
Volume 581, Issue 22, Pages 4279-4287Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2007.07.079
Keywords
actin remodelling; cell migration; F-actin interaction; phosphorylation leading edge
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Protein kinase D (PKD) has been implicated in the regulation of cell shape, adhesion, and migration. At the leading edge of migrating cells active PKD co-localizes with F-actin, Arp3 and cortactin. Platelet derived growth factor (PDGF) activates PKD and recruits the kinase to the leading edge, suggesting a role for PKD in actin remodelling. In support of this, PKD directly interacts with F-actin and phosphorylates cortactin in vitro. Interference with PKD function by overexpression of a dominant negative PKD or by PKD-specific siRNA enhanced cell migration, whereas cells overexpressing PKD wild type displayed reduced migratory potential. Taken together, these data reveal a negative regulatory function of PKD in cell migration. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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