Journal
OPTICS LETTERS
Volume 32, Issue 18, Pages 2641-2643Publisher
OPTICAL SOC AMER
DOI: 10.1364/OL.32.002641
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We demonstrate that both oxyhemoglobin and deoxyhemoglobin have sequential two-color, two-photon absorption properties that can serve as endogenous contrasts in microvasculature imaging. Using a sensitive modulation transfer technique, we are able to image hemoglobin in red blood cells with micrometer resolution, both in vitro and in vivo. We show that excellent contrast from hemoglobin without any labeling can be obtained in tissue. (c) 2007 Optical Society of America.
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